Feyond多功能酶标仪助力论文发表

文章：A New Producer of Echinocandins, Ascomycete Coleophoma sp. Isolated from the Lichen Stereocaulon paschale

发表单位：Russian Academy of Sciences

期刊：Microbiology

IF值：1.3    

中科院分区：Q4

检测应用：菌生长曲线

具体方法：To determine the effect of the extract on the growth kinetics of test cultures (Yarrowia lipolytica VKM Y-3322, Candida parapsilosis S134, Candida albicans T5 and T6), we used the method of cultivation in immunological 96-well plates using a multimodal reader Feyond-A300 (China).

文章：Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

发表单位：江南大学

期刊：Scientific Reports

IF值：3.8    

中科院分区：Q2

检测类型：荧光

检测应用：钙离子

具体方法：The spectrofluorometer (Feyond-A300, All Sheng Co. Ltd., Hangzhou, China) was used to assess the fluorescence of Fluo-4-Ca2+ at excitation at 488 nm and emission in the range of 512–520 nm. Data was documented at intervals of 10 s.

文章：The efficient activity of plant essential oils for inhibiting Botrytis cinerea and Penicillium expansum: Mechanistic insights into antifungal activity

发表单位：Universidad de La Frontera

期刊：Microbiological Research

IF值：6.1    

中科院分区：Q1

检测类型：荧光

检测应用：真菌生物量与生物质的干重

具体方法：The fluorescence intensity was measured in a multi-modal microplate reader (Feyond-a300,Allsheng). The arbitrary fluorescence units were corrected according to the absorbance at 450 nm, assuming that fungal biomass was correlated linearly with the dry weight of the biomass.

文章：Multivariate Modular Metabolic Engineering for High Titer Uridine Triphosphate Production in Escherichia coli

发表单位：江南大学

期刊：ACS Sustainable Chemistry & Engineering

IF值：7.1    

中科院分区：Q1

检测类型：荧光

检测应用：GFP蛋白

具体方法：Samples were taken as required and were further diluted 1:5 in 200 μL of fresh LB with antibiotics and inducers for the measurement using a Feyond-A300 microplate reader (All Sheng Co. Ltd., Hangzhou, China). GFP abundance was quantified at an excitation wavelength of 488 ± 10 nm and an emission wavelength of 511 ± 10 nm.

文章：A simple method for effective cryopreservation of antlerogenic periosteum of sika deer

发表单位：长春理工大学

期刊：Journal of Experimental Zoology-A

IF值：1.9    

中科院分区：Q3

检测类型：吸收光

检测应用：CCK8细胞活性

具体方法：The cells were seeded in 96‐well‐plates at density of 2000 cells/well. When the cells reached around 60% confluence, the medium was replaced by 90 μL new complete medium and 10 μL CCK‐8 solution/ well for further 2 h. Subsequently, the solution was measured at 450 nm using a microplate reader (Feyond‐A300).

文章：p38 and ERK1/2-Dependent Activation of c-Jun Is Required for the Downregulation of Oxidative Stress-Induced ERα in Hypothalamic Astrocytes

发表单位：福建医科大学协和医院

期刊：Neuroendocrinology

IF值：3.2    

中科院分区：Q2

检测类型：荧光

检测应用：ROS

具体方法：The results are expressed by the fluorescence value, which was measured using an excitation wavelength of 480 nm and emission wavelength of 525 nm (Feyond- A300)

文章：Ilyomycin L, a novel resorcylic acid lactone with furan nucleus from Ilyonectria sp. DWS906

发表单位：中南大学湘雅医院老年疾病国家临床医学研究中心药学系

期刊：Journal of Molecular Structure

IF值：4    

中科院分区：Q2

检测类型：吸收光

检测应用：CCK8细胞活性

具体方法：

Finally, 10 μL of CCK-8 was added to each well for 1–4h, and the absorbance was measured at 450 nm using a microplate

reader (Feyond-A300, allsheng, Hangzhou, China).

文章：The mechanistic insights of essential oil of Mentha piperita to control Botrytis cinerea and the prospection of lipid nanoparticles to its application

发表单位：Universidad de La Frontera

期刊：Microbiological Research

IF值：6.1    

中科院分区：Q1

检测类型：荧光

检测应用：

MitoTracker? Orangepropidium iodide、Calcein-AM、CellROX Deep Red、TPA

具体方法：

Furthermore, the fluorescence intensity was determined in a multimodal microplate reader (Feyond-a300, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). The following excitation and emission wavelengths were used in this study: 554/575 nm for MitoTracker? Orange, 535/617 nm for propidium iodide, 477/520 nm for Calcein-AM, and 350/432 for Calcofluor White.

A standard curve of H2O2 (SigmaAldrich?, St. Louis, MO, USA) was measured from 1 μM to 200 μM tointerpolate de obtained optical density. The following excitation and emission wavelengths were used in this study: 645/675 nm for CellROX Deep Red, 320/425 nm for TPA.

文章：Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

发表单位：福建医科大学第一附属医院

期刊：Analytical Methods

IF值：2.7    

中科院分区：Q3

检测类型：吸收光

检测应用：光谱检测

具体方法：

The UV-vis absorption spectra were recorded using a microplate reader (Feyond-A300).

The color of the detection system was recorded by a phone camera. The UV-vis absorbance spectra were measured using a microplate reader

文章：Macrogenomics-Based Analysis of the Effects of Intercropped Soybean Photosynthetic Characteristics and Nitrogen-Assimilating Enzyme Activities on Yield at Different Nitrogen Levels

发表单位：吉林大学

期刊：Microorganisms

IF值：4.1    

中科院分区：Q2

检测类型：吸收光

检测应用：CCK8

具体方法：

The activity titre was determined using an enzyme label (Feyond-A300, 0–4 OD, ALLSHENG, Shanghai, China) at respective wavelengths of 340 nm, 540 nm, 340 nm, 630 nm, and 240 nm.

文章：Examination of the relationship of carbonate alkalinity stress and ammonia metabolism disorder-mediated apoptosis in the Chinese mitten crab, Eriocheir sinensis: Potential involvement of the ROS/MAPK signaling pathway

发表单位：中国科学院黑龙江水产研究所

期刊：Aquaculture

IF值：3.9    

中科院分区：Q1

检测类型：荧光

检测应用：蛋白定量

具体方法：Finally, the fluorescence of hemocytes was measured with a fluorescence microplate reader (Feyond-A300) at an ex/em wavelength of 470/525 nm,

文章：Silver nanoparticles induce endothelial cytotoxicity through ROS-mediated mitochondria-lysosome damage and autophagy perturbation: The protective role of N-acetylcysteine

发表单位：宁夏医科大学总医院

期刊：Toxicology

IF值：4.8    

中科院分区：Q3

检测类型：发光

检测应用：ATP

具体方法：ATP content was measured using a multifunctional microplate reader (Feyond-A300, AllSheng Instruments, Hangzhou City, Zhejiang, China), expressed as the number of relative light units (RLUs), and normalized to the protein concentration

文章：Purification, characterization, and antioxidant ability of polysaccharides from Phascolosoma esculentas

发表单位：宁德师范学院

期刊：Food Science & Nutrition

IF值：3.5    

中科院分区：Q2

检测类型：荧光

检测应用：ROS

具体方法：Thereafter, C. elegans were mixed with DCFH-DA (20 μM) and then reacted for 2 h without light. Subsequently, C. elegans were transferred to a glass slide and photographed by fluorescence microscope (DM6B, Leica, Germany). Additionally, the relative fluorescence intensity was measured by an enzyme-labeled instrument (Feyond-A300, Shanghai, China).

文章：Macrocyclic trichothecenes from Myrothecium verrucaria PA 57 and their cytotoxic activity

发表单位：中南大学湘雅医院

期刊：Chinese Journal ofNatural Medicines

IF值：4    

中科院分区：Q2

检测类型：吸收光

检测应用：CCK8细胞活性

具体方法：

Finally, 10 μL of CCK-8 was added to each well for 1?4 h, and the absorbance was detected at 450 nm with a microplate reader (Feyond- A300, allsheng, Hangzhou, China). The GraphPad Prism 9.5.1 software was adopted to calculate IC50 values.

文章：Maize/Soybean Intercropping with Straw Return Increases Crop Yield by Influencing the Biological Characteristics of Soil

发表单位：吉林农业大学

期刊：Scientific Reports

IF值：4.1    

中科院分区：Q2

检测类型：吸收光

检测应用：酶活

具体方法：Their activity was determined using an enzyme-labeling instrument (Feyond- A300, 0-4 OD, Aosens, Hangzhou, China) at wavelengths of 630, 240, 508, and 600 nm, respectively

文章：NUDT21 interacts with NDUFS2 to activate the PI3K/AKT pathway and promotes pancreatic cancer pathogenesis

发表单位：首都医科大学宣武医院

期刊：Journal of Cancer

IF值：3.3    

中科院分区：Q3

检测类型：吸收光

检测应用：NADP + /NADPH

具体方法：In particular, NADP + / NADPH ratios were determined using a NADP + /NADPH Assay kit (S0179, Beyotime) and were detected using a colorimetric assay under a microplate reader (Feyond-A400, ALLSHENG, China) with detection wavelengths of 570 nm, respectively.

文章：OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death

发表单位：首都医科大学宣武医院

期刊：Cell Death Discovery

IF值：6.1    

中科院分区：Q2

检测类型：吸收光

检测应用：NADP + /NADPH

具体方法：In particular, NADP+/NADPH ratios were determined using a NADP+/NADPH Assay kit (S0179, Beyotime) and were detected using a colorimetric assay under a microplate reader (Feyond-A400, ALLSHENG, China) with detection wavelengths of 570 nm, respectively.