双孔隙通道（TPC）

双孔通道（TPC）是位于细胞内体上的钙通道。由第二信使NAADP和磷脂磷脂磷脂酰肌醇3,5-二磷酸，PtdIns（3,5）P2）激活，孔隙主要传导Ca2+和Na+。已经证明，它们在埃博拉病毒感染中起着关键作用，并可能作为埃博拉病毒和其他丝状病毒的抗病毒研究提供研究靶点。在内质体膜中，它们负责埃博拉病毒的进入。

Key Publications

Sakurai, Y., Kolokoltsov, A.A., Chen, C-C., et al. 2015. Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment. Science

Castonguay, J., Orth, J.H.C., Müller, T., et al. 2017. The two-pore channel TPC1 is required for efcient protein processing through early and recycling endosomes. Nature Scientific Reports

Measuring Two Pore Channels

Two Pore Channels can be measured using automated patch clamp when expressed in cell lines, in lysosomes, or using the Orbit instruments when they are recombinantly expressed in bilayers.

Automated patch clamp

Parallel bilayer recordings

Cytotoxicity measurements
Publications using Instruments from Nanion
2017 - Organelle membrane derived patches: reshaping classical methods for new targets
2014 - High susceptibility to fatty liver disease in two-pore channel 2-deficient mice
2010 - Planar Patch Clamp Approach to Characterize Ionic Currents from Intact Lysosomes
2010 - Characterization of two pore channel 2 (TPCN2) -mediated Ca2+ currents in isolated lysosomes