资源编号：BNCC353367
英文名： Homo sapiens, human│colon
形态： epithelial
提供形式： frozen
安全等级： 1
应用领域： This cell line is a suitable transfection host. This line has a mutation in codon 13 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.
培养方法
| 培养基 | The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 传代方法 | Volumes are given for a 75 cm2 flask. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days |
| 生长条件 | Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CGrowth Conditions: Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts. |
| 生长特性 | adherent |
| 存储条件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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