Recombinant Human TGF-alpha Protein,hFc Tag

蛋白别名（Synonyms）

ETGF; TGF-alpha; TGF type 1; TGFA

种属（Species)

Human

表达系统（Source）

HEK293

表达序列（Sequence）

Val40-Ala89 

Accession

P01135-1

标签（Tag)

N-hFc

分子量（Molecular Weight）

The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA 

SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

外观（Appearance）

冻干粉