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性能参数
|
表达区间及表达系统(Source) |
Human GPA Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ser20-Glu91.[Accession | P02724-1] |
|
分子量大小(Molecular Weight) |
The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result. |
|
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
|
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性(Activity) |
SPR Data: Anti-GPA Antibody immobilized on CM5 Chip can bind Human GPA, hFc Tag with an affinity constant of 0.72 μM as determined in SPR assay |
|
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230625
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