Recombinant Human CA9/Carbonic Anhydrase IX (His-Avi Tag)

Uniprot No.

Q16790

表达区间及表达系统

Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln38-Asp414.

分子量

Approximately 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data: Immobilized Human CA9, His Tag at 0.9μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA.

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.