Recombinant Human NRG1-β2 Protein

Product Name

Cat#

Size

Recombinant Human NRG1-β2 Protein

92710ES10

10 μg

92710ES50

50 μg

92710ES60

100 μg

92710ES76

500 μg

92710ES80

1 mg

Synonyms

Heregulin-beta1, HRG1

Accession

Q02297

GeneID

3084

Source

E.coli-derived Human NRG1-β2, Ser177-Gln237.

Molecular Weight

Approximately 7.0 kDa.

AA Sequence

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 10⁵ U/mg. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.