Recombinant Rat IL-4 Protein

Synonyms

Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra

Accession

P20096

GeneID

287287

Source

E.coli-derived rat IL-4 protein, His23-Ser147.

Molecular Weight

Approximately 14.0 kDa.

AA Sequence

HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.