Recombinant Human VEGFR2/KDR(mFc Tag)

Uniprot No.

P35968-1

表达区间及表达系统（Source）

Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 Cells with mFc tag at the C-terminal. It contains Ala20-Glu764.

分子量(Molecular Weight)

Approximately 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human VEGF165 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC₅₀ of 36.1 ng/mL determined by ELISA.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.