Recombinant Human Tβ-4

Accession

P62328

GeneID

7114

Source

E.coli-derived human Tβ-4 protein, Ser2-Ser44.

Molecular Weight

Approximately 4.9 kDa.

AA Sequence

SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 μg/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.