Rat Eotaxin/CCL11

Accession

P97545

GeneID

29397

Source

E.coli-derived Rat Eotaxin/CCL11, His24-Pro97.

Molecular Weight

Approximately 8.4 kDa.

AA Sequence

HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/mL. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.