Recombinant Human TWEAK Receptor/TNFRSF12A

Accession

Q9NP84

GeneID

51330

Source

E.coli-derived Human TWEAK Receptor/TNFRSF12A protein,Glu28-Pro80.

Molecular Weight

Approximately 5.6 kDa

AA Sequence

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 10⁴ IU/mg, in the presence of 15 ng/mL of rHuTWEAK. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.