TGFB1人重组蛋白

蛋白名称:TGFB1人重组蛋白，10804-H08H

蛋白构建:A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.

表达宿主:HEK293 Cells

蛋白纯度:> 95 % as determined by SDS-PAGE

蛋白活性:

1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His (Cat: 10804-H08H) at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc (Cat: 10358-H03H), the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL.

2.Loaded Recombinant Human TGF-beta RII/TGFBR2 Protein, hFc Tag (Cat. No. 10358-H02H ) on ProA Biosensor, can bind Recombinant Human TGF beta 1 Protein (Latent), His Tag (Cat. No. 10804-H08H) with an affinity constant of 47.1 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).

蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method

预测N端:Leu 30 & Ala 279

蛋白分子量:The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.

蛋白NP号:NP_000651.3

蛋白氨基酸序列:Met1-Ser390

蛋白标签:C-His

欢迎去义翘神州网查看TGFB1人重组蛋白，10804-H08H详细信息。网址：https://cn.sinobiological.com/recombinant-proteins/human-tgf-beta-1-10804-h08h

义翘神州研发团队拥有多年的重组蛋白研发经验。多年来，我们的团队已开发出超过9300余种重组蛋白，涵盖免疫检查点、抗体药物靶点、CAR-T细胞治疗靶点、Fc受体、流感病毒蛋白以及细胞因子等热门研究领域。义翘神州重组蛋白具有高纯度、高生物活性和低内毒素水平等特性，可为生物药靶点发现、蛋白质结构和功能分析、细胞疗法和重组酶酶学性质等科学研究提供支持。

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