人非小细胞肺癌细胞|BNCC245633|冻存管；T25培养瓶

产品中文名称

人非小细胞肺癌细胞

产品英文名称

NCI-H1568[H1568]

形态特性

上皮细胞样，多角形，边缘不规则

培养基

88%RPMI-1640+10%FBS+1%谷氨酰胺+1%丙酮酸钠

培养条件

37℃；5%CO2+95%空气；

培养方法

复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心5min，弃去上清液，用1-2mL完全培养基重悬细胞。③将细胞悬液加入到含有5-6mL完全培养基的T25瓶中，放入培养箱培养。
细胞传代：①将旧培养液吸除，PBS清洗两遍后，加入1-2mL胰酶（0.25%Trypsin+0.02%EDTA）；②镜下观察消化情况，在细胞边缘缩小，贴壁松动时（可用吸管吸起些许胰酶轻轻吹打细胞层某处，肉眼可见细胞层脱落，即消化完成，否则继续消化），直接吸掉胰酶，加入5-6mL完全培养基，轻轻吹打细胞层，把细胞层吹落，吹散。③将细胞悬液按1:2比例分到新的T25瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养。；④注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到80%-90%时，重复传代操作或者冻存。

存储形式

液氮

提供形式

冻存管；T25培养瓶

备注

‖Age│48 years‖Gender│female‖Ethnicity│Caucasian‖Derivation│The line was established in December 1986 from lymph node metastasis of lung.‖Clinical Data│48 years Caucasian female The patient was a smoker. 60 pack years.‖STR Profile│Amelogenin X
CSF1PO 11
D13S317 11
D16S539 10,12
D5S818 9
D7S820 8,9
THO1 9
TPOX 8,11
vWA 16,17‖Year of Origin│1986‖Restrictions│ The line is available with the following restrictions 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.