人髓母细胞瘤细胞

人髓母细胞瘤细胞介绍：

产品中文名称：人髓母细胞瘤细胞
产品英文名称：D341 Med
参考用途：/
形态特性：上皮细胞样，圆形，多数聚团
培养基：80%EMEM+20%FBS
培养条件：37℃；5%CO2+95%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心3-5min，弃去上清液，用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中，放入培养箱培养。培养瓶建议竖着培养。
细胞传代：①离心法：收集细胞，1000rpm离心5min，弃去上清液，补加1-2mL培养液后吹匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。②半量换液法：可选择半量换液方式；请将上清培养基轻轻吸走一半，将剩余留有细胞沉淀的培养基重悬混匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到大于2×106个/mL时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│3.5 years‖Gender│male‖Karyotype│This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.‖Derivation│The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.‖Clinical Data│The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
male‖Genes Expressed│The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.‖Cellular Products│neurofilament proteins‖Tumorigenic│Yes‖Effects│Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors
Yes, in soft agar‖STR Profile│Amelogenin X,Y
CSF1PO 10,11
D13S317 11,13
D16S539 12,14
D5S818 11,12
D7S820 9,13
F13A01 6,7
F13B 8,10
FESFPS 10,13
LPL 11
THO1 6,9.3
TPOX 8,11
vWA 17,18‖Population Doubling Time│37 hrs‖Year of Origin│1988‖描述│The cells produce tumors in nude mice.
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
The c-myc oncogene is amplified in D341 Med cells.