大肠杆菌（含pARO181质粒）

大肠杆菌（含pARO181质粒）介绍：

注意：该价格为冻干粉价格
产品中文名称：大肠杆菌（含pARO181质粒）
产品英文名称：Escherichia coli（pARO181）
参考用途：expression vector
vector containing primer sites useful for sequencing
vector permitting visual detection of recombinants
vector useful for insertional mutagenesis
形态特性：大小：1-2mm 形状：圆形 边缘：整齐 透明度：不透明 颜色：白色 隆起度：中间凸起 表面：明亮光滑 质地：湿润易挑起
培养基：酵母膏：5.0g，蛋白胨：10.0g，NaCl：10.0g，pH：7.0(25℃)
培养条件：37°C；18-24h；好氧
培养方法：①准备1支含有5~10mL液体培养基的试管和2个平板；②安全柜中打开，用酒精灯灼烧顶部，后迅速滴上无菌水使之破裂，随后用镊子将其敲碎；③吸取0.5mL液体培养基打入冻干管，充分溶解后重新打回液体试管中，混匀；④吸取0.2mL菌悬液打入平板中，涂布均匀，重复两次获得两个平板；⑤将液体试管和平板全部置于上述培养条件下培养，菌种长出即可使用。
存储形式：2-8℃
提供形式：冻干粉
备注：‖Vector Information│Size (kb) 6.0700001716613770
Vector pARO181 (plasmid)
Promoters Promoter lac
Construction pUC18, pSUP2021, Tn5
Marker(s)kanR
Construct size (kb) 6.070000171661377
Features insert detection lacZ'marker(s) kanRpromoter lacreplicon pMB1‖Shipping Information│Frozen glycerol stock of E. coli containing the plasmid‖描述│Restriction digests of the clone give the following sizes (kb): HindIII/PvuI--6.0; PvuI--6.0; EcoRI/BglII--3.7, 2.3; EcoRI/PvuI--6.0.
Mobilizable expression vector encoding the lacZalpha (lacZ') peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing.
Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains.
Replicates to high copy number in E. coli and Enterobacter aerogenes.
Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4.
The order of the major features in this plasmid is: lacZ'/MCS - pMB1 ori - kanR - oriT/bom. pARO181 (ATCC 77125) and pARO191 (77126) differ in the orientation of the MCS in lacZ'.