人免疫球蛋白lambda骨髓瘤细胞|BNCC270394|冻存管；T25培养瓶

人免疫球蛋白lambda骨髓瘤细胞介绍：

产品中文名称：人免疫球蛋白lambda骨髓瘤细胞
产品英文名称：MM.1S
参考用途：CRL-2974和CRL-2975共同提供了有关疾病进展和耐药性发展的重要信息。这些细胞系为阐明糖皮质激素的作用机制和开发新的治疗方法提供了有价值的工具。
形态特性：淋巴母细胞样，圆形，单个细胞
培养基：90%RPMI-1640+10%FBS
培养条件：37℃；5%CO2+95%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心3-5min，弃去上清液，用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中，放入培养箱培养。培养瓶建议竖着培养。
细胞传代：①离心法：收集细胞，1000rpm离心5min，弃去上清液，补加1-2mL培养液后吹匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。②半量换液法：可选择半量换液方式；请将上清培养基轻轻吸走一半，将剩余留有细胞沉淀的培养基重悬混匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到大于2×106个/mL时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│42 years RefGreenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol. 31271-282, 2003. PubMed; 12691914‖Gender│female‖Ethnicity│Black‖Derivation│The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R (ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone.‖Clinical Data│Black
female
42 years‖Antigen Expression│CD25+, CD38+, CD52+, CD59+ RefGreenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol. 31271-282, 2003. PubMed; 12691914‖Receptor Expression│glucocorticoid receptor, expressed‖Genes Expressed│lambda-light chain immunoglobulin‖Cellular Products│lambda-light chain immunoglobulin‖STR Profile│D5S818 8, 12
D13S317 13
D7S820 10, 11
D16S539 12
vWA 15, 17
THO1 7, 8
TPOX 8
Amelogenin X
CSF1PO 9,14‖Population Doubling Time│approximately 72 hours‖Year of Origin│1986‖描述│MM.1S is sensitive to dexamethasone.