人小细胞肺癌细胞

人小细胞肺癌细胞介绍：

产品中文名称：人小细胞肺癌细胞
产品英文名称：NCI-H146 [H146]
参考用途：/
形态特性：淋巴母细胞样，圆形，单个细胞，多数聚团
培养基：88%RPMI-1640+10%FBS+1%谷氨酰胺+1%丙酮酸钠
培养条件：37℃；5%CO2+95%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心3-5min，弃去上清液，用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中，放入培养箱培养。培养瓶建议竖着培养。
细胞传代：①离心法：收集细胞，1000rpm离心5min，弃去上清液，补加1-2mL培养液后吹匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。②半量换液法：可选择半量换液方式；请将上清培养基轻轻吸走一半，将剩余留有细胞沉淀的培养基重悬混匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到大于2×106个/mL时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│59 years‖Gender│male‖Ethnicity│Caucasian‖Karyotype│This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.‖Clinical Data│59 years
Caucasian
male‖Receptor Expression│insulin-like growth factor II (IGF II)‖Oncogene│myc +; myb +; raf +; ras +; fms +; fes -‖Genes Expressed│The cells do not express vasopressin, oxytocin or gastrin releasing peptide. The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.
There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
Oncogenes myc +; myb +; raf +; ras +; fms +; fes -‖Tumorigenic│Yes‖Effects│Yes, in nude mice‖STR Profile│Amelogenin X
CSF1PO 11
D13S317 11,12
D16S539 11
D5S818 12
D7S820 9,10
THO1 6,9.3
TPOX 8,11
vWA 14,16‖Isoenzymes│AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1-2
PGM3, 1-2‖Year of Origin│1979‖Restrictions│ The line is available with the following restrictions This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harr