人急性T淋巴细胞白血病细胞

人急性T淋巴细胞白血病细胞介绍：

产品中文名称：人急性T淋巴细胞白血病细胞
产品英文名称：MOLT-3
参考用途：
形态特性：淋巴母细胞样，圆形，单个细胞，少数聚团
培养基：90%RPMI-1640+10%FBS
培养条件：37℃；5%CO2+95%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心3-5min，弃去上清液，用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中，放入培养箱培养。培养瓶建议竖着培养。
细胞传代：①离心法：收集细胞，1000rpm离心5min，弃去上清液，补加1-2mL培养液后吹匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。②半量换液法：可选择半量换液方式；请将上清培养基轻轻吸走一半，将剩余留有细胞沉淀的培养基重悬混匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到大于2×106个/mL时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│19 years‖Gender│male‖Karyotype│This is a hypertetraploid human cell line. The modal chromosome number was 97, occurring in 48% of cells. The rate of cells with higher ploidies was 2%. Three markers were common to all cells. They are ?del(6) (q21), t(7;7), and an unidentifiable M5. The der(6) generally had three copies per cell, and the i(7q), two copies. The majority of normal chromosomes had 4 copies per cell. N8, N17, N19 and N20 had more than 4 copies in many cells. The X and Y chromosomes were two copies each per cell. Neither HSR chromosomes nor DM's were detected.‖Derivation│MOLT-3 was derived from the same patient as the MOLT-4 cell line (ATCC CRL-1582).
This line was established from cells taken from a patient in relapse.‖Clinical Data│male
19 years‖Antigen Expression│CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%)‖Genes Expressed│terminal deoxynucleotidyl transferase (TdT) activity is high‖Cellular Products│terminal deoxynucleotidyl transferase (TdT) activity is high‖Isoenzymes│AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0‖描述│No immunoglobulin or Epstein-Barr virus is detectable.
The patient had received prior multidrug chemotherapy.