pTracer-CMV2

质粒类型：哺乳动物表达载体

启动子：CMV

载体大小：6210 bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Zeocin (博莱霉素)

pTracer-CMV2是一个双表达框载体，CMV启动子后有MCS供插入外源基因；hEF1α启动子调控Cycle 3 GFP-Zeocin fusion的表达，可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰，而且Zeocin也可以用来做稳定性细胞筛选。另外，由于EM-7启动子的存在，Cycle 3 GFP-Zeocin fusion在E.coli中也可以表达，可以用来做原核荧光定位。 

Three pTracer mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin fusion. 

All three pTracer vectors have the following features:

（1）Cycle 3 GFP-Zeocin fusion for selection in mammalian cell lines

（2）BGH polyA signal and transcription termination sequences to enhance mRNA stability 

The pTracer-CMV2 vector offers:

（1）CMV promoter for high-level constitutive expression of your gene of interest

（2）EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin fusion

（3）Ampicillin resistance gene for selection in E. coli