pIEX-Bac1

质粒类型：昆虫细胞表达系统

启动子：IE1, p10

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：6796 bp 

载体标签：C-His,N-Strep

载体抗性：Ampicillin (氨苄青霉素)

备注：拥有hr5增强子

The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.

The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.