pNZ8148

启动子：PnisA promoter

复制子：repA，repC

终止子：T

质粒分类：乳酸杆菌表达载体；乳酸乳球菌表达载体

质粒大小：3167 bp

原核抗性：Chl+（氯霉素），工作浓度为25ug/ml

克隆菌株：MC1061

培养温度：37℃

表达宿主：NZ9000乳酸乳球菌

备注：注意pNZ8148转化扩增、克隆构建时必须使用MC1061感受态。您可以从HonorGene购买获得MC1061菌株。

The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII. 

The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus. 

pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).