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Cdc42-GTP 小鼠单抗
- 品牌：美国NewEast Biosciences
- 货号： 26905
- CAS编号：
- 产地：武汉
- 供应商报价： 面议 （市场参考价：¥ 4800）
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武汉费斯德生物科技有限公司
更新时间：2023-04-16 23:55:39 -
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- 详细介绍
货号: 26905 规格: 30 µL 基因符号: CDC42 描述: Anti-Cdc42-GTP Monoclonal Antibody 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). 免疫原: Recombinant full length protein of active Cdc42 经过测试的应用: IP, IHC 推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: Mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG1 纯度: Purified from ascites 防腐剂: No 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-Cdc42-GTP monoclonal antibody recognizes active Cdc42 from vertebrates. 储存条件: Store at -20°C. Avoid repeated freezing and thawing IP-蛋白质印迹:
IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008).
免疫荧光:
Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld’s hard set mounting medium (H-1500), and examined using a Zeiss inverted fluorescence microscope.
- 主要用途
- GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
