P0364 pBC-Hygro

P0364 pBC-Hygro 20μl "启动子: cpc-1
复制子: pUC ori，F1 ori
终止子: trpC
质粒分类: 其他宿主穿梭载体；丝状真菌表达质粒
质粒大小: 6817bp
质粒标签: LacZ
原核抗性: 氯霉素chloramphenicol
筛选标记: 潮霉素Hygromycin
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 丝状真菌
5'测序引物: M13F：TGTAAAACGACGGCCAGT
3'测序引物: M13R：CAGGAAACAGCTATGACC
质粒简介
pBC-hygro是一个丝状真菌表达载体，酶切位点有ApaI，XhoI，SalI，ClaI，HindIII，EcoRI，SmaI，SpeI，XbaI，NotI。

质粒图谱

质粒序列
LOCUS       Exported                6817 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pBC-hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2016-02-20 from MiaoLingBio http://www.miao领bio.com
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
            http://www.miao领bio.com
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism=""synthetic DNA construct""
                     /mol_type=""other DNA""
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /note=""f1 ori""
                     /note=""f1 ori;f1 ori;f1 bacteriophage origin of
                     replication; arrow indicates direction of (+) strand
                     synthesis""
     CDS             complement(241..816)
                     /codon_start=1
                     /gene=""lacZ fragment""
                     /product=""LacZ-alpha fragment of beta-galactosidase""
                     /note=""lacZ""
                     /note=""lacZ fragment;lacZ-alpha""
                     /translation=""MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG
                     FPRQALNRGLPLGFRFSALRHLDPKKLD""
     primer_bind     600..616
                     /note=""M13 fwd""
                     /note=""M13 fwd;M13 fwd;common sequencing primer, one of
                     multiple similar variants""
     promoter        626..644
                     /note=""T7 promoter""
                     /note=""T7 promoter;T7 promoter;promoter for bacteriophage
                     T7 RNA polymerase""
     misc_feature    653..760
                     /note=""MCS""
                     /note=""MCS;MCS;pBluescript multiple cloning site""
     primer_bind     670..686
                     /note=""KS primer""
                     /note=""KS primer;KS primer;common sequencing primer, one of
                     multiple similar variants""
     primer_bind     complement(720..736)
                     /note=""SK primer""
                     /note=""SK primer;SK primer;common sequencing primer, one of
                     multiple similar variants""
     promoter        complement(773..791)
                     /note=""T3 promoter""
                     /note=""T3 promoter;T3 promoter;promoter for bacteriophage
                     T3 RNA polymerase""
     primer_bind     complement(812..828)
                     /note=""M13 rev""
                     /note=""M13 rev;M13 rev;common sequencing primer, one of
                     multiple similar variants""
     protein_bind    836..852
                     /bound_moiety=""lac repressor encoded by lacI""
                     /note=""laco""
                     /note=""lac repressor encoded by lacI lac operator;lac
                     operator;The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).""
     promoter        complement(860..890)
                     /note=""lac promoter""
                     /note=""lac promoter;lac promoter;promoter for the E. coli
                     lac operon""
     protein_bind    905..926
                     /bound_moiety=""E. coli catabolite activator protein""
                     /note=""CAP""
                     /note=""E. coli catabolite activator protein CAP bindin;CAP
                     binding site;CAP binding activates transcription in the
                     presence of cAMP.""
     rep_origin      complement(1214..1802)
                     /direction=LEFT
                     /note=""ori""
                     /note=""ori;ori;high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication""
     promoter        2022..2124
                     /note=""cat promoter""
                     /note=""cat promoter;cat promoter;promoter of the E. coli
                     cat gene encoding chloramphenicol acetyltransferase""
     CDS             2125..2784
                     /codon_start=1
                     /gene=""cat""
                     /product=""chloramphenicol acetyltransferase""
                     /note=""Chl""
                     /note=""cat;CmR;confers resistance to chloramphenicol""
                     /translation=""MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA""
     misc_feature    3732..4535
                     /note=""CPC-1 Promoter""
     CDS             4541..5566
                     /codon_start=1
                     /gene=""aph(4)-Ia""
                     /product=""aminoglycoside phosphotransferase from E. coli""
                     /note=""aph""
                     /note=""aph(4)-Ia;HygR;confers resistance to hygromycin""
                     /translation=""MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E""
     misc_feature    5567..6501
                     /note=""trpC Terminator""
     promoter        complement(6689..6794)
                     /gene=""bla""
                     /note=""bla bla AmpR promoter""
                     /note=""bla AmpR promoter;AmpR promoter""
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
      781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcgaccgaa
     1921 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
     1981 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt
     2041 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttgtcgaga
     2101 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     2161 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     2221 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     2281 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     2341 ttacgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     2401 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     2461 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2521 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2581 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc