P0768 pDL11

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P0768 pDL11 20μl "基本信息
启动子: alcA
质粒分类: 黑曲霉表达质粒
质粒标签: lacZ, argB
原核抗性: 氨苄青霉素Ampicillin
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 黑曲霉菌
质粒简介
pDL11是一个黑曲霉表达质粒.
质粒序列
>LacZ-R 5'-GACAGTATCGGCCTCAGGAA-3'
CACNCGCTTCGGCCCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACT
GTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAG
GCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACCATGGTGAGGCGAGGT
GATAGGATTGGAAGAGTTCTAATTAACTGAGTAGAGAACTGTTGATTGTTGGTTGATGATGTTGGTGAGA
CTGAGAACCTTGGGGGTCTTTATATAGATGTTCAGCTATGCGGGGATGCGATCCTGGGGTAGGAGAGCAC
GTACGGGGCCCCGCTCGTTTGTGGCTCTCCGTGCGGACATCCCGTGCGGACAGTACCAGAAAGTGCTCCG
TCTCTGCTCTATACGGCTCTATACGCGTACCTCTTGAACGGTGCGTGGAGAGGAGTGGTGTGTCAATTTC
CGCCCCGCCCTCGTGCGGTTCCGCATGCATCCAATCCTAGGTCGGAACTATCCCGAGCTGCGGATGCCGA
TGCGGACGGACAAGTGGGAACTATCACAATCAGCTTTTCAGAATTCGAGCTCGGTACCCGGGGACNCCNC
TGCCTNNNCCATTGCNAAACCTCAGAAAATGTGCTGGATAGAGGTCAAGAGTATGGAATGAGGAGATCNA
GGTTGGGTGTAGCGNCTGNNTCTCTTGGCAGAACCCCCCCTCNGAACTGNAANCCCGGGGNTANNCCTTC
TCATTNCCNATTCAAACCTNATATTANGACNAAANTGCATAATATGAANGTA"
P0385 pIB139 20μl "基本信息
启动子: ermEp
复制子: pBR322 ori
质粒分类: 链霉菌表达质粒
质粒标签: LacZα
原核抗性: 安普霉素（阿伯拉霉素）Apr
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 链霉菌
5'测序引物: 参考相关文献
质粒简介
文献：max.book118.com/html/2016/0201/34460972.shtm
质粒图谱

质粒序列
APR测序序列：
        1 aagttcgagc tgctcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
       61 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
      121 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
      181 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
      241 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
      301 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
      361 ggggcaaggt cgcgctgatt gctggggcaa cacgtggagc ggatcgggga ttgtctttct
      421 tcagctcgct gatgatatgc tgacgctcaa tgccgtttgg cctccgacta acgaaaatcc
      481 cgcatttgga cggctgatcc gattggcacg gcggacggcg aatggcggag cagacgctcg
      541 tccgggggca atgagatatg aaaaagcctg aactcaccgc gacgtatcgg gccctggcca
      601 gctagctaga gtcgacctgc aggtccccgg ggatcggtct tgccttgctc gtcggtgatg
      661 tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca
      721 atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg
      781 gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag
      841 ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt
      901 cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc
      961 atagtccacg acgcccgtga ttttgaaccc tggccgacgg ccagcaggta ggccgacagg
     1021 tcatgccggc cgcggccgct tttcctcatc gtcttcg"
P0386 pOJ446 20μl "复制子: pUC ori
质粒分类: 链霉菌系列质粒
质粒大小: 10.4kb
原核抗性: 安普霉素（阿伯拉霉素）Apr
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 链霉菌
质粒简介

 pOJ446质粒相关文献
点击下载
质粒图谱

质粒序列
APR测序序列：
        1 ttctctgctc ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc
       61 attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag
      121 cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc
      181 ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa
      241 ctgggccgag atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat
      301 gccgctcgcc agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc
      361 aaggtcgcgc tgattgctgg ggcaacacgt ggagcggatc ggggattgtc tttcttcagc
      421 tcgctgatga tatgctgacg ctcaatgccg tttggcctcc gactaacgaa aatcccgcat
      481 ttggacggct gatccgattg gcacggcgga cggcgaatgg cggagcagac gctcgtccgg
      541 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
      601 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
      661 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
      721 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
      781 gtgcttgaca ttggggaatt ctacttcgtc gaggactggc gcatccgcgg ttcccctctt
      841 ggatg"
P0480 pJTU1278 20μl "复制子: pIJ101 ori,ColE1 ori，RP4 oriT
质粒分类: 链霉素系列质粒
质粒大小: 9237bp
原核抗性: 氨苄青霉素Ampicillin
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 链霉菌
质粒图谱
"
P0802 p5921 20μl 质粒分类: 其他宿主载体；白色念珠菌表达质粒
P0803 pCaEXP 20μl 质粒分类: 其他宿主载体；白色念珠菌表达质粒
P0900 pSET152 20μl 质粒分类: 其他宿主载体；链霉菌基因整合过表达质粒

VT7102 pAN8-1 20μl "启动子: gpdA
复制子: pUC ori
终止子: TRPC
质粒分类: 其他宿主载体；丝状真菌表达质粒
质粒大小: 6089bp
原核抗性: 氨苄青霉素Ampicillin
筛选标记: 博来霉素Bleocin(Zeocin)
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 丝状真菌
诱导方式: 无需诱导
5'测序引物: 根据全序列设计
3'测序引物: 根据全序列设计
备注: NCBI号：Z32751.1
质粒简介
          pAN8-1, encoding phleomycin resistance. Protoplasts of a vigorous Phleor transformant were then retransformed using the partially homologous vector, pAN7-1 which encodes hygromycin B resistance. Retransformation of this strain to hygromycin resistance occurred at frequencies that were consistently twofold higher than with the original recipient strain. Linearised pAN7-1 DNA transformed phleomycin-resistant protoplasts at higher frequencies still.

质粒图谱

质粒序列
LOCUS       Exported                6089 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAN8-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
            http://www.miao领bio.com
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism=""synthetic DNA construct""
                     /mol_type=""other DNA""
     misc_feature    1..2129
                     /note=""gpdA Promoter""
     misc_feature    2178..2293
                     /note=""Intron""
     CDS             2302..2676
                     /codon_start=1
                     /gene=""Sh ble from Streptoalloteichus hindustanus""
                     /product=""antibiotic-binding protein""
                     /note=""Zeo""
                     /note=""confers resistance to bleomycin, phleomycin, and
                     Zeocin(TM)""
                     /translation=""MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD""
     misc_feature    complement(2733..3456)
                     /note=""trpC terminator""
     primer_bind     complement(3464..3480)
                     /note=""M13 fwd""
                     /note=""common sequencing primer, one of multiple similar
                     variants""
     promoter        3954..4058
                     /gene=""bla""
                     /note=""AmpR promoter""
     CDS             4059..4919
                     /codon_start=1
                     /gene=""bla""
                     /product=""beta-lactamase""
                     /note=""AmpR""
                     /note=""confers resistance to ampicillin, carbenicillin, and
                     related antibiotics""
                     /translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW""
     rep_origin      5090..5678
                     /direction=RIGHT
                     /note=""ori""
                     /note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication""
     protein_bind    5966..5987
                     /bound_moiety=""E. coli catabolite activator protein""
                     /note=""CAP binding site""
                     /note=""CAP binding activates transcription in the presence
                     of cAMP.""
     promoter        6002..6032
                     /note=""lac promoter""
                     /note=""promoter for the E. coli lac operon""
     protein_bind    6040..6056
                     /bound_moiety=""lac repressor encoded by lacI""
                     /note=""lac operator""
                     /note=""The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).""
     primer_bind     6064..6080
                     /note=""M13 rev""
                     /note=""common sequencing primer, one of multiple similar
                     variants""
ORIGIN
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc

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