pNOY136, p100KB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因

pNOY122, pSRP107 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY122, pSRP107     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8167 联系人：郭经理   订购电话：15910316995  QQ：3579779715订购**：​www.biovector.net
pNOY123, pSRP108 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY123, pSRP108     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8168 联系人：郭经理   订购电话：15910316995  QQ：3579779716订购**：www.biovector.net
pNOY124, pSRP109 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY124, pSRP109     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8169 联系人：郭经理   订购电话：15910316995  QQ：3579779717订购**：www.biovector.net
pNOY125, pSRP110 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY125, pSRP110     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8170 联系人：郭经理   订购电话：15910316995  QQ：3579779718订购**：www.biovector.net
pNOY126, pSRP111 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY126, pSRP111     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8171 联系人：郭经理   订购电话：15910316995  QQ：3579779719订购**：www.biovector.net
pNOY127, pSRP112 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY127, pSRP112     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8172 联系人：郭经理   订购电话：15910316995  QQ：3579779720订购**：www.biovector.net
pNOY128, pSRP114 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY128, pSRP114     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8173 联系人：郭经理   订购电话：15910316995  QQ：3579779721订购**：www.biovector.net
pNOY129, pSRP115 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY129, pSRP115     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The plasmids (pNOY118-pNOY129) containing different DNA fragments of NSM1(SRP1) region but all of the common HpaI-BglII fragment of NSM1-1(SRP1-1). These NSM1(SRP1) gene fragments have the suppressor mutation NSM1-1(SRP1-1). The clones were selected from the Gene Library which was constructed with genomic DNA of NOY343. Partially digested DNA by Sau3AI were cloned into BamHI site of pNOY6 [=pYCp50 (URA3+, ARSI+, CEN4, Ampr), Ryoji Yano BYP8174 联系人：郭经理   订购电话：15910316995  QQ：3579779722订购**：www.biovector.net
pNOY130, pNOY102+5S 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY130, pNOY102+5S     【载体类型Vector Type】：YEp     【插入基因Inserted Gene】： 5S rDNA, 37S rDNA     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: 356 bp EcoRI/PvuII of pBB111R (=pNOY 3133) containing the 5S gene. The EcoRI end was treated with Klenow enzyme to End-fill before clonning into the SmaI site of pNOY102, 16,450 bp, Denis Heck BYP8175 联系人：郭经理   订购电话：15910316995  QQ：3579779723订购**：www.biovector.net
pNOY131, pSRP107-B 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY131, pSRP107-B     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: 4.8 kb ClaI fragment including NSM1-1 (SRP1-1) gene cut out from pSRP107 (pNOY122) was cloned into ClaI site of pUN35 (pNOY63). This fragment contains BamHI-ClaI fragment isolated from YCp50 (pNOY6) next to NSM1-1 (SRP1-1), Ryoji Yano BYP8176 联系人：郭经理   订购电话：15910316995  QQ：3579779724订购**：www.biovector.net
pNOY132, pSRP107-C 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY132, pSRP107-C     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: SmaI-HpaI fragment (~2.5 kb) including NSM1-1 (SRP1-1) cut out from pSRP107B (pNOY131) and cloned into SmaI site of pUN35 (pNOY63), Ryoji Yano BYP8177 联系人：郭经理   订购电话：15910316995  QQ：3579779725订购**：www.biovector.net
pNOY133, p103KS 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY133, p103KS     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： upstream and downstream of NSM1 (SRP1)    【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The KpnI-HpaI (~2 kb) fragment upstream of NSM1 gene and the BglII-SacI (0.8 kb) fragment downstream of NSM1 gene were cloned into KpnI-EcoRV and BamHI-SacI sites of pRS316, respectively. NSM1 gene was cut out from pSRP103 (pNOY119). This plasmid is for cloning of wild type gene of NSM1 with transformation after cutting by SmaI. vector::pRS316 (pNOY73) (URA+, AmpR), Ryoji Yano BYP8178 联系人：郭经理   订购电话：15910316995  QQ：3579779726订购**：www.biovector.net
pNOY134, pSRP100 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY134, pSRP100     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: The original clone of NSM1(SRP1) wild type gene. This plasmid was isolated following transformation of NOY260 with p103KS (pNOY133)(gap repair method). Vector::pRS316 (pNOY73) (URA3+, AmpR), Ryoji Yano BYP8179 
pNOY135, p100HS 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY135, p100HS     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: pSRP100 (pNOY134) was cut by KpnI and HpaI. KpnI was blunt ended by Klenow enzyme then ligated with HpaI site. This plasmid has HpaI-SacI fragment of NSM1(wild type) gene. Vector::pRS316 (pNOY73) (URA3+, AmpR), Ryoji Yano BYP8180 联系人：郭经理   订购电话：15910316995  QQ：3579779728订购**：www.biovector.net
pNOY136, p100KB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY136, p100KB     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: KpnI-BglII fragment isolated from pSRP100 (pNOY134) and cloned into KpnI-BamHI site of pNOY71 (pRS314). Vector::pRS314 (pNOY71) (TRP1+, Amp+), Ryoji Yano BYP8181