pNOY176, 306-4-11KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种

pNOY157, pY21-14 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY157, pY21-14     【载体类型Vector Type】：YEp     【插入基因Inserted Gene】： RPA12'     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: RPA12 gene on a 0.6 kb BamHI-PstI fragment on pTV3 (pNOY161). The carboxy-terminal 21 amino acids of RPA12 gene of pNOY155, 156 and 157 are deleted by this construction. (BamHI site was ereated by deletion from HindIII site and BamHI linker attachment), Y. Nogi BYP8202 
pNOY158, pY21-30 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY158, pY21-30     【载体类型Vector Type】：YEp     【插入基因Inserted Gene】： rpa12:: LEU2     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： LEU2     【其他Comments】: LEU2 gene (2.2 kb) flanked by BamHI and PstI replaced ScaI-PstI region of RPA12 to disrupt RPA12. ScaI site was converted to BamHI site. This fusion fragment was cloned to between HindIII and SmaI sites of YEp351. To disrupt RPA12, cut pNOY158 with HindIII and XhoI to get the 3.2 kb fragment transform into yeast, Y. Nogi BYP8203 联系人：郭经理   订购电话：15910316995  QQ：3579779751订购**：www.biovector.net
pNOY159, pY21-17 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY159, pY21-17     【载体类型Vector Type】：YEp     【插入基因Inserted Gene】： RPA12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： LEU2     【其他Comments】: BamHI linker (CGGATCCG) is inserted at ScaI site of RPA12 gene on pNOY154, Y. Nogi BYP8204
pNOY160, pY21-ura3 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY160, pY21-ura3     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： RPA12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3, TRP1     【其他Comments】: 1.1 kb HIndIII URA3 gene fragment from YEp24 (pNOY77) was cloned into SmaI site of pNOY153. (HindIII sites were blunt-dnded by Klenow), Y. Nogi BYP8205 
pNOY162, pNSM1-TRP1 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY162, pNSM1-TRP1     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: The 2.4 kb KpnI-SalI (converted to blunt end) of SRP1 gene isolated from pUC100HB (pNOY3152) was cloned into KpnI-SmaI sites of pRS314 (pNOY71). This is the original plasmid for mutant isolation, R. Yano BYP8206 联系人：郭经理   订购电话：15910316995  QQ：3579779754订购**：www.biovector.net
pNOY163, 3-1PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY163, 3-1PE     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-11, (3-1PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. PstI-EcoRI fragment on pNSM1-TRP1 (pNOY162) was replaced with mutant fragment, R. Yano BYP8207 
pNOY164, 3-24PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY164, 3-24PE     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-12, (3-24PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. PstI-EcoRI fragment on pNSM1-TRP1 (pNOY162) was replaced with mutant fragment, R. Yano BYP8208 
pNOY165, 4-7KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY165, 4-7KP     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-13, (4-7KP)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. The KpnI-PstI fragment on pNOY162 was replaced with mutant fragment, R. Yano BYP8209
pNOY166, 4-9PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY166, 4-9PE     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-14, (4-9PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. PstI-EcoRI fragment on pNSM1-TRP1 (pNOY162) was replaced with mutant fragment, R. Yano BYP8210 联系人：郭经理   订购电话：15910316995  QQ：3579779758订购**：www.biovector.net
pNOY167, 4-11KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY167, 4-11KP     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-15, (4-11KP)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. The KpnI-PstI fragment on pNOY162 was replaced with mutant fragment, R. Yano BYP8211 
pNOY168, 5-4EB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY168, 5-4EB     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-16, (5-4EB)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. The EcoRI-BamHI fragment on pNOY162 was replaced with mutant fragment, R. Yano BYP8212 ​
pNOY169, 5-11KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY169, 5-11KP     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-17, (5-11KP)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. The KpnI-PstI fragment on pNOY162 was replaced with mutant fragment, R. Yano BYP8213
pNOY170, 6-15KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY170, 6-15KP     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-18, (6-15KP)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. The KpnI-PstI fragment on pNOY162 was replaced with mutant fragment, R. Yano BYP8214 联系人：郭经理   订购电话：15910316995  QQ：3579779762订购**：www.biovector.net
pNOY171, 7-43PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY171, 7-43PE     【载体类型Vector Type】：YCp     【插入基因Inserted Gene】： srp1-19, (7-43PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： TRP1     【其他Comments】: This plasmid is SRP1 Ts mutant plasmid on which the fragment with mutation was replaced in the wild type gene. The original plasmid is pNOY162 (pNSM1-TRP1). The mutant was isolated as Ts mutant after hydroxyamine treatment. PstI-EcoRI fragment on pNSM1-TRP1 (pNOY162) was replaced with mutant fragment, R. Yano BYP8215
pNOY172, 306-3-1PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY172, 306-3-1PE     【载体类型Vector Type】：YIp     【插入基因Inserted Gene】： srp1-11, (3-1PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: This plasmid is SRP1 ts mutant plasmid with integration vector and has the same mutant gene fragment as in pNOY163. The vector was used is pRS306 (pNOY69), the integration vector with URA3 maker, R. Yano BYP8216 联系人：郭经理   订购电话：15910316995  QQ：3579779764订购**：www.biovector.net
pNOY173, 306-3-24PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY173, 306-3-24PE     【载体类型Vector Type】：YIp     【插入基因Inserted Gene】： srp1-12, (3-24PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: This plasmid is SRP1 ts mutant plasmid with integration vector and has the same mutant gene fragment as in pNOY164. The vector was used is pRS306 (pNOY69), the integration vector with URA3 maker, R. Yano BYP8217 联系人：郭经理   订购电话：15910316995  QQ：3579779765订购**：www.biovector.net
pNOY174, 306-4-7KP 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY174, 306-4-7KP     【载体类型Vector Type】：YIp     【插入基因Inserted Gene】： srp1-13, (4-7KP)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: This plasmid is SRP1 ts mutant plasmid with integration vector and has the same mutant gene fragment as in pNOY165. The vector was used is pRS306 (pNOY69), the integration vector with URA3 maker, R. Yano BYP8218 联系人：郭经理   订购电话：15910316995  QQ：3579779766订购**：www.biovector.net
pNOY175, 306-4-9PE 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY175, 306-4-9PE     【载体类型Vector Type】：YIp     【插入基因Inserted Gene】： srp1-14, (4-9PE)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： URA3     【其他Comments】: This plasmid is SRP1 ts mutant plasmid with integration vector. It has the same mutant gene fragment as in pNOY166. The vector was used is pRS306 (pNOY69), the integration vector with URA3 maker, R. Yano BYP8219 联系人：郭经理   订购电话：15910316995  QQ：3579779767订购**：www.biovector.net