SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE质粒载体
pUC18(质粒载体)|Beyotime/碧云天|1μg
pUC18(质粒载体)|Beyotime/碧云天|100μg
细胞基因质粒载体
细胞基因质粒载体
pNOY3088 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3088 【载体类型Vector Type】: 【插入基因Inserted Gene】: lacZ::RPA190 【大肠杆菌筛选标记E.coli selection marker】: 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: EcoRI/BamHI fragment from pNOY3063 containing RPA190 gene cloned into EcoRI/BamHI cut pNO1575 puts lacZ/RPA190 fusion under ? promoter control. First 17 amino acids of RPA190 replaced by 11 amino acids from lacZ multiple cloning site (MCS in pNO1575 derived from pUC9), Mike Wittekind BYP8698 联系人:郭经理 订购电话:15910316995 QQ:3579780243订购**:www.biovector.net
pNOY3089 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3089 【载体类型Vector Type】: 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: No description BYP8699 联系人:郭经理 订购电话:15910316995 QQ:3579780244订购**:www.biovector.net
pNOY3090 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3090 【载体类型Vector Type】: 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: No description BYP8700 联系人:郭经理 订购电话:15910316995 QQ:3579780245订购**:www.biovector.net
pNOY3091 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3091 【载体类型Vector Type】: 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: No description BYP8701 联系人:郭经理 订购电话:15910316995 QQ:3579780246订购**:www.biovector.net
pNOY3092 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3092 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: RPA190 【大肠杆菌筛选标记E.coli selection marker】: Cm 【酵母筛选标记S.pombe selection marker】: TRP1 【其他Comments】: Integrative plasmid ~10 kb BglII fragment from pNOY19 containing wild type RPA190 gene and TRP1 gene (not ARS1) cloned into BamHI site of pACYC184. Chloramphenicol resistant and complements trpC E. coli host, Mike Wittekind BYP8702 联系人:郭经理 订购电话:15910316995 QQ:3579780247订购**:www.biovector.net
pNOY3093 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3093 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: rpa190-1 【大肠杆菌筛选标记E.coli selection marker】: Cm 【酵母筛选标记S.pombe selection marker】: TRP1 【其他Comments】: Same as pNOY3092, but BglII fragment derived from pNOY23 (rpa190-1 ts). Same orientation as pNOY3092, Mike Wittekind BYP8703 联系人:郭经理 订购电话:15910316995 QQ:3579780248订购**:www.biovector.net
pNOY3094 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3094 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: rpa190-2 【大肠杆菌筛选标记E.coli selection marker】: Cm 【酵母筛选标记S.pombe selection marker】: TRP1 【其他Comments】: Same as pNOY3092 but BglII fragment derived from pNOY24 (rpa190-2 ts). Same orientation as pNOY3092, Mike Wittekind BYP8704 联系人:郭经理 订购电话:15910316995 QQ:3579780249订购**:www.biovector.net
pNOY3095 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3095 【载体类型Vector Type】: 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: SalI/BamHI CEN3 fragment from pGALCEN (pNOY49) cloned into SalI/BamHI site of pIB121 (pNO2929), Mike Wittekind BYP8705 联系人:郭经理 订购电话:15910316995 QQ:3579780250订购**:www.biovector.net
pNOY3096, YIp351 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3096, YIp351 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: LEU2 【其他Comments】: Integrative plasmid containing LEU2 gene and multiple cloning site from pUC18, Mike Wittekind BYP8706 联系人:郭经理 订购电话:15910316995 QQ:3579780251订购**:www.biovector.net
pNOY3097, YIp352 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3097, YIp352 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: URA3 【其他Comments】: Integrative plasmid containing URA3 gene and multiple cloning site from pUC18 (see ref for pNOY3096), Mike Wittekind BYP8707 联系人:郭经理 订购电话:15910316995 QQ:3579780252订购**:www.biovector.net
pNOY3098 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3098 【载体类型Vector Type】: 【插入基因Inserted Gene】: RPA190 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: XhoI/BglII fragment containing RPA190 gene cloned into XhoI/SalI sites of pNOY3095. Done by cutting pNOY16 DNA with BglII and fil领 in with Klenow with only dGTP and dATP. Then cut with XhoI and RPA190 fragment isolated on Sea Plaque Agarose. Vector pNOY3095 cut with SalI then treated with Klenow with only dCTP and dTTP, then cut with XhoI and ligated with RPA190 fragment, Mike Wittekind BYP8708 联系人:郭经理 订购电话:15910316995 QQ:3579780253订购**:www.biovector.net
pNOY3100 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3100 【载体类型Vector Type】: 【插入基因Inserted Gene】: SpURA4 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: S. pombe Ura4 gene (ca 1.8 kb HindIII frag.) was inserted into HindIII site of pUC8, Jan Kohl, M. Yamamoto BYP8709 联系人:郭经理 订购电话:15910316995 QQ:3579780254订购**:www.biovector.net
pNOY3101 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3101 【载体类型Vector Type】: 【插入基因Inserted Gene】: Ty1 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: Ty-plasmid contains yeast Ty1 gene inserted into pBR322 as 5.4 kb EcoRI insert, AmpR, Jan Kohl BYP8710 联系人:郭经理 订购电话:15910316995 QQ:3579780255订购**:www.biovector.net
pNOY3105 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3105 【载体类型Vector Type】: 【插入基因Inserted Gene】: rDNA 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: BamHI (in G12 parent)-BglII fragment carrying yeast rDNA (rDNA region: spacer & promoter) was cloned into BamHI site of pBluescript II KS+. Use as PolI transcription template, Dan Riggs BYP8711 联系人:郭经理 订购电话:15910316995 QQ:3579780256订购**:www.biovector.net
pNOY3106 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3106 【载体类型Vector Type】: 【插入基因Inserted Gene】: rDNA 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: BamHI (in G12 parent)-XbaI fragment cloned into the same sites of pBluescript II KS+. Insert : yeast rDNA with spacer, 5S gene and promoter, Dan Riggs BYP8712 联系人:郭经理 订购电话:15910316995 QQ:3579780257订购**:www.biovector.net
pNOY3113, pKmX11 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3113, pKmX11 【载体类型Vector Type】: 【插入基因Inserted Gene】: rDNA 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: G418 【其他Comments】: 1.4 kb PvuII of pNOY74 (YCpG11) encoding G418R gene (KmR gene) was inserted into the 3598 bp XmnI site of pNOY3106. The direction of transcription of G418 gene is opposite to rRNA transcription, 8.7 kb, Yas Nogi BYP8713 联系人:郭经理 订购电话:15910316995 QQ:3579780258订购**:www.biovector.net
pNOY3114, pUC-LEU2A 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3114, pUC-LEU2A 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: LEU2 【其他Comments】: 2.7 kb BglII fragment encoding the LEU2+ gene from YEp13 (pNOY2) was ligated into BamHI site of pUC19, Ryoji Yano BYP8714 联系人:郭经理 订购电话:15910316995 QQ:3579780259订购**:www.biovector.net
pNOY3115, pUC-LEU2B 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3115, pUC-LEU2B 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: LEU2 【其他Comments】: Same construct as pNOY3114 but LEU2+ in opposite orientation, Ryoji Yano BYP8715 联系人:郭经理 订购电话:15910316995 QQ:3579780260订购**:www.biovector.netpNOY3116, pKS300 出芽酵母载体质粒BioVector NTCCZG
pNOY3119 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3119 【载体类型Vector Type】: 【插入基因Inserted Gene】: UBC8 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: Entire codeing region of UBC8 is inserted right downstream of T7 promoter and rbs of pT7-7 (see Current Protocols in Molecular Biology, 1989), Qin Siliang BYP8717 联系人:郭经理 订购电话:15910316995 QQ:3579780262订购**:www.biovector.net
pNOY3120, pSRP305 ks 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3120, pSRP305 ks 【载体类型Vector Type】: 【插入基因Inserted Gene】: SRP3-1 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: SalI-BamHI fragment (7 kb) including SRP3-1 cut out from pNOY84 (pSRP305B) was ligated into the SalI-BamHI site on pBluescript KS-. This plasmid has the same fragment of yeast DNA as pNOY84, Ryoji Yano BYP8718 联系人:郭经理 订购电话:15910316995 QQ:3579780263订购**:www.biovector.net
pNOY3121, p305LEU 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3121, p305LEU 【载体类型Vector Type】: 【插入基因Inserted Gene】: rpa135Δ::LEU2 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: 【其他Comments】: RPA135 gene is disrupted by substitution with LEU2+ gene. XhoI-BamHI fragment can be used for RPA135 disruption. XhoI site in LEU2+ fragment was disrupted, vector : pBluescript II KS+, Ryoji Yano BYP8719 联系人:郭经理 订购电话:15910316995 QQ:3579780264订购**:www.biovector.net
pNOY3122, pYN1002 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3122, pYN1002 【载体类型Vector Type】:YIp 【插入基因Inserted Gene】: tRNA (Glu) 【大肠杆菌筛选标记E.coli selection marker】: Amp 【酵母筛选标记S.pombe selection marker】: URA3 【其他Comments】: 3.0 kb EcoRI fragment complementing ts-E27 (ts-E78) was subcloned into EcoRI site of YIp352 (pNOY3097). XhoI-EcoRV contains tRNA (Glu). See pNOY111 and pNOY112, Note: Yas sent this plasmid to Bayer (Seattle) as pNOY3121, Yas Nogi BYP8720
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已咨询173次质粒载体
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已咨询154次基因/cDNA库
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已咨询133次质粒载体
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