pNOY3150, T7-RRN4 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞

pNOY3129, pYS701 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3129, pYS701     【载体类型Vector Type】：    【插入基因Inserted Gene】： GAL7     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: GAL7 fragment from BamHI to SalI (~1.4 kb) was cloned into pSP64, Yas Nogi BYP8721 联系人：郭经理   订购电话：15910316995  QQ：3579780266订购**：www.biovector.net
pNOY3131, p613RV 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3131, p613RV     【载体类型Vector Type】：    【插入基因Inserted Gene】： partial rDNA     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: 613 bp SmaI/EcoRV from pNO3106 was cloned into EcoRV digested pBluescript KS+ to aid yield of rDNA probe region, Denis Heck BYP8722 联系人：郭经理   订购电话：15910316995  QQ：3579780267订购**：www.biovector.net
pNOY3132, p961-5 or p961-6 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3132, p961-5 or p961-6     【载体类型Vector Type】：    【插入基因Inserted Gene】： URA3     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: 961 bp HindIII-PstI of pNOY102 was inserted into the same sites of pBluescript to increase yield of URA3 DNA probe fragment, Denis Heck BYP8723 联系人：郭经理   订购电话：15910316995  QQ：3579780268订购**：www.biovector.net
pNOY3134, pProm (PvuII) 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3134, pProm (PvuII)     【载体类型Vector Type】：    【插入基因Inserted Gene】： 5S rDNA     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: PvuII fragment of rDNA from upstream of 5S gene (at -1478) to downstream of PolI promoter (at + 582) inserted into SmaI site of vector. Note that it contains the 5S gene. Orientation is such that RNAP1 transcribes through the KpnI site in MCS. Parent : pNOY3106, Vector : pBluescript II KS+, Use : RNAP I Transcription Initiation Template, Dan Riggs BYP8724 联系人：郭经理   订购电话：15910316995  QQ：3579780269订购**：www.biovector.net
pNOY3138 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3138     【载体类型Vector Type】：    【插入基因Inserted Gene】： rpa135-11     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: rpa135-11 on pBluescript II KS- (pNOY3048). Same construction as pNOY3116 except the mutation rpa135-11 of the first Cys -> Ala (TGT -> GCT) at Zn-finger site. This gene was made by site-specific mutagenesis (ref: Methods in Enzymol. 154:367-382, 1987), H. Sonoda BYP8725 联系人：郭经理   订购电话：15910316995  QQ：3579780270订购**：www.biovector.net
pNOY3139 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3139     【载体类型Vector Type】：    【插入基因Inserted Gene】： rpa135-12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: rpa135-12 on pNOY3048, same as pNOY3116 except the mutation rpa135-12 of the second Cys -> Ala at the Zn-finger region (TGT -> GCT), H. Sonoda BYP8726 联系人：郭经理   订购电话：15910316995  QQ：3579780271订购**：www.biovector.net
pNOY3140 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3140     【载体类型Vector Type】：    【插入基因Inserted Gene】： rpa135-13     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: rpa135-13 on pNOY3048, same as pNOY3116 except the mutation rpa135-13 of the third Cys -> Ala at the Zn-finger region (TGC -> GCC), H. Sonoda BYP8727 联系人：郭经理   订购电话：15910316995  QQ：3579780272订购**：www.biovector.net
pNOY3141, pSRP103HB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3141, pSRP103HB     【载体类型Vector Type】：    【插入基因Inserted Gene】： NSM1-1 (SRP1-1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: HpaI-BglII fragment cut out from pSRP103 (pNOY119) was cloned into EcoRV-BamHI sites of pBluescript II KS-. This NSM1 (SRP1) gene has suppressor mutation (NSM1-1 or SRP1-1), Ryoji Yano BYP8728 联系人：郭经理   订购电话：15910316995  QQ：3579780273订购**：www.biovector.net
pNOY3142, p103LEU 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3142, p103LEU     【载体类型Vector Type】：    【插入基因Inserted Gene】： NSM1Δ ::LEU2     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: PstI-EcoRV fragment of p103HB (inside of NSM1) was substituted with PstI-SmaI fragment of pNOY3114. This plasmid is for making disrupted mutant of NMS1. vector :: pBluescript II KS-, Ryoji Yano BYP8729 联系人：郭经理   订购电话：15910316995  QQ：3579780274订购**：www.biovector.net
pNOY3143, pNOY3152, p100HB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3143, pNOY3152, p100HB     【载体类型Vector Type】：    【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: HpaI-BglII fragment of NSM1 (SRP1) cut out from pSRP100 was cloned into SmaI-BamHI sites of pUC19, Ryoji Yano BYP8730 联系人：郭经理   订购电话：15910316995  QQ：3579780275订购**：www.biovector.net
pNOY3144, pT7-102 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3144, pT7-102     【载体类型Vector Type】：    【插入基因Inserted Gene】： T7p-NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】：     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: The coding region of NSM1 (SRP1) wt gene was ligated into NdeI-BamHI site of pJes307 (pNO3095). The initiation codon of NSM1 was positioned at the initiation codon of T7 gene, so intact NSM1 protein is prodused in E. coli with this plasmid. NdeI was created at the initiation site of NSM1. Ryoji Yano BYP8731 ​
pNOY3145, p100C-lacZ 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3145, p100C-lacZ     【载体类型Vector Type】：    【插入基因Inserted Gene】： lacZ-'NSM1     【大肠杆菌筛选标记E.coli selection marker】：     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: DraI-BglII fragment of NSM1 (C-terminal end), ~10 kb part of NSM1, was ligated into EcoRI-SalI sites of pT7-lacZ (pNO3096). This is for making lacZ fusion protein of NSM1 to make anti-serum, Ryoji Yano BYP8732 联系人：郭经理   订购电话：15910316995  QQ：3579780277订购**：www.biovector.net
pNOY3146 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3146     【载体类型Vector Type】：    【插入基因Inserted Gene】：     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: pCITE -1 vector , Ryoji Yano BYP8733 联系人：郭经理   订购电话：15910316995  QQ：3579780278订购**：www.biovector.net
pNOY3147, pY21-8 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3147, pY21-8     【载体类型Vector Type】：YIp     【插入基因Inserted Gene】： RPA12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】： LEU2     【其他Comments】: 3.2 kb BamHI-XhoI fragment containing RPA12 gene was cloned between BamHI and XhoI sites of pRS305. To integrate this gene, pNOY3147 was cut at PstI (unique site), Yas Nogi BYP8734
pNOY3148, TrpE-RRN4 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3148, TrpE-RRN4     【载体类型Vector Type】：    【插入基因Inserted Gene】： trpE-RPA12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: BamHI site of pATH22 was cut, repaired and ligated (ΔBamHI-ATH22). 8 bp (CGGATCCG) of BamHI linker was inserted at SmaI site of ΔBamHI-ATH22. Then, the 0.6 kb BamHI-XhoI fragment containing RPA12 was inserted between BamHI and SalI sites of the above vector. BamHI site was created at the ScaI site of RPA12 gene by insertion of the 8 bp BamHI linker, Yas Nogi BYP8735 联系人：郭经理   订购电话：15910316995  QQ：3579780280订购**：www.biovector.net
pNOY3149, GEX-RRN4 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3149, GEX-RRN4     【载体类型Vector Type】：    【插入基因Inserted Gene】： GST-RPA12     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: 0.6 kb BamHI-XhoI fragment containing RPA12 was inserted between BamHI and SmaI sites of pGEX-3x after the XhoI site was blunt ended with Klenow, Yas Nogi BYP8736 联系人：郭经理   订购电话：15910316995  QQ：3579780281订购**：www.biovector.net
pNOY3152, pNOY3143, p100HB 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3152, pNOY3143, p100HB     【载体类型Vector Type】：    【插入基因Inserted Gene】： NSM1 (SRP1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: The HpaI-BglII fragment (2.4 kb) of SRP1 (wt) was cloned into pUC19. SmaI-BamHI sites, Ryoji Yano BYP8738 联系人：郭经理   订购电话：15910316995  QQ：3579780283订购**：www.biovector.net
pNOY3154, pHIS-SRP1 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3154, pHIS-SRP1     【载体类型Vector Type】：    【插入基因Inserted Gene】： His tag-SRP1     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: HpaI-Sau3AI (388 bp) fragment including SRP1 promoter was cloned into HpaI-BglII site on pET-15b (pNOY3151), and NdeI-BglII (1949 bp) fragment from p100NdeI (pNOY139) was also cloned into NdeI-BamHI sites, Ryoji Yano BYP8739 联系人：郭经理   订购电话：15910316995  QQ：3579780284订购**：www.biovector.net
pNOY3155, pUC18-SRP1 E/B 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3155, pUC18-SRP1 E/B     【载体类型Vector Type】：    【插入基因Inserted Gene】： nsm1 (srp1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: The EcoRI-BamHI (1 kb) fragment of SRP1 cut out from pNOY162 was cloned into EcoRI-BamHI sites of pUC18. This is the template for PCR reactions of pUC300 PCR (pNOY3156), pUC600 PCR (pNOY3157) and pUC720 PCR (pNOY3158), Ryoji Yano BYP8740 联系人：郭经理   订购电话：15910316995  QQ：3579780285订购**：www.biovector.net
pNOY3156, pUC300PCR 出芽酵母载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏中心Budding yeast vector pNOY3156, pUC300PCR     【载体类型Vector Type】：    【插入基因Inserted Gene】： nsm1 (srp1)     【大肠杆菌筛选标记E.coli selection marker】： Amp     【酵母筛选标记S.pombe selection marker】：     【其他Comments】: The EcoRI-SmaI (RY11-oligo) PCR product was cloned into EcoRI-SmaI sites of pUC19 (see pNOY183, pNOY184), Ryoji Yano BYP8741 联系人：郭经理   订购电话：15910316995  QQ：3579780286订购**：www.biovector.net