Recombinant Rhesus macaque CD4/LEU3 Protein,His Tag

表达区间及表达系统（Source）

Rhesus macaque CD4/LEU3 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Lys26-Trp390.[Accession | G7N5T8]

分子量大小（Molecular Weight）

The protein has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

ELISA Data: Immobilized Rhesus macaque CD4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.