Recombinant Human APOE/Apolipoprotein E(His Tag)

Uniprot No.

P02649-1

表达区间及表达系统（Source）

Recombinant Human APOE / Apolipoprotein E Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Lys19-His317.

分子量(Molecular Weight)

Approximately 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE

活性（Activity）

1. SPR Data: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 1.004 μM as determined in SPR assay (Biacore T200).

2. SPR Data: Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 115.9 nM as determined in SPR assay (Biacore T200).

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.