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Recombinant Human IL-27 Protein,His Tag

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详细介绍

性能参数

分子别名（Synonyms）	IL30;IL-27A;p28;Interleukin-27
表达区间及表达系统（Source）	Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus. It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).[Accession \| Q14213(IL-27B)&Q8NEV9(IL-27A)]
分子量大小（Molecular Weight）	The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE
活性（Activity）	ELISA Data: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA .
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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