Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein,His-Avi Tag

表达区间及表达系统（Source）

Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala46-Pro217.

[Uniprot | P31994-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

SPR Data: Biotinylated Human Fc gamma RIIB, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 μM as determined in SPR assay.

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100

μg/ml is recommended. Dissolve the lyophilized protein in distilled water.