Recombinant Human Sigle10(hFc-Avi Tag)

Uniprot No.

Q96LC7-1

表达区间及表达系统（Source）

HEK293 Cells-derived Human Siglec-10 Protein, Met17-Thr546 with hFc tag and Avi tag at the C-terminal

分子量(Molecular Weight)

Approximately 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human Siglec-10 at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC₅₀ of 14.4 ng/mL determined by ELISA.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.