Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein,His-Avi Tag

表达区间及表达系统（Source）

Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg20-Pro189(IL-23 alpha)&Ile23-Ser328(IL-12 beta).[Accession | Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)]

分子量大小（Molecular Weight）

The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE

活性（Activity）

ELISA Data: Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA  

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.