Recombinant Biotinylated Human NKG2A&CD94 Protein,His-Avi Tag

表达区间及表达系统（Source）

Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)]

分子量大小（Molecular Weight）

The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Anti-NKG2A Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0.11μg/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.