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当前位置:仪器网>产品中心> 翌圣生物科技(上海)股份有限公司>抗体>一抗>Cyclin B1 Rabbit pAb 兔多克隆抗体
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Cyclin B1 Rabbit pAb 兔多克隆抗体

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详细介绍

该基因编码的蛋白质是一种参与有丝分裂的调节蛋白。 基因产物与p34(cdc2)形成促成熟因子(MPF)。 已经发现了两种替代转录本,一种是组成型表达转录本,另一种是细胞周期调控转录本,主要在G2/M期表达。 不同的转录产物是由不同的转录起始位点所决定的。

 

产品性质

别名

CCNB1; CCNB; G2/mitotic-specific cyclin-B1

SwissProt ID

P14635

基因ID

891

免疫原

The antiserum was produced against synthesized peptide derived from human Cyclin B1. AA range:91-140

缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3

分子量

Observed MW (kDa):60

克隆类型

Polyclonal Antibody

亚型

IgG

宿主

Rabbit

物种反应

Human,Mouse,Rat

纯化方式

Affinity Purified

细胞定位

细胞质,细胞核

研究领域

细胞生物学与发育生物学

 

应用

稀释比WB: 1/500-1/2000 IHC: 1/100-1/300 IF: 1/200-1/1000 ELISA: 1/20000

 


image.png

Western Blot analysis of Cyclin B1 in PC-12 cells using Cyclin B1 Polyclonal Antibody diluted at 1/50

image.png

Immunofluorescence analysis of Cyclin B1 in mouse liver tissue. 1,Cyclin B1 Polyclonal Antibody(red) was diluted at 1:200 (4°C,overnight).

2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).

3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

 

image.png

Immunofluorescence analysis of Cyclin B1 in rat liver tissue. 1,Cyclin B1 Polyclonal Antibody(red) was diluted at 1:2 (4°C,overnight).

2, Cy3 labled Secondary antibody was diluted at 1:3 (room temperature, 5min).

3, Picture B: DAPI(blue) 1min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

 

image.png

Immunohistochemical analysis of paraffin-embedded human tonsil tissue.

1,Cyclin B1 Polyclonal Antibody was diluted at 1:200

(4°C,overnight).

2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min).

3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

 

 

运输与保存方法

冰袋运输-20存,1年有效期

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

HB220607

 

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