Recombinant Human ICOS/CD278 (C136S, C137S) Protein,His Tag

分子别名（Synonyms）

CRP-1; CD278;AILIM; ICOS

表达区间及表达系统（Source）

Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu21-Phe141(C136S,C137S).[Accession | Q9Y6W8-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA .

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.