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Recombinant Cynomolgus GARP&Latent TGF beta 1 Complex Protein,His-Avi Tag

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详细介绍

性能参数

分子别名（Synonyms）	LAP; GARP;LRRC32; LRRC32&TGF-beta 1
表达区间及表达系统（Source）	Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).[Accession \| A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1)]
分子量大小（Molecular Weight）	The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta 1) based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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