Recombinant Human 4-1BB/TNFRSF9(His Tag)

别名（Synonyms）

CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA

Uniprot No.

Q07011

表达区间及表达系统（Source）

HEK293 Cells-derived Human 4-1BB/TNFRSF9 Protein, Leu24-Gln186 with His tag at the C-terminal

分子量(Molecular Weight)

Approximately 18.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human 4-1BB, His Tag at 1μg/mL (100 μL/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC₅₀ of 5.7 ng/mL determined by ELISA.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.