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Recombinant Human DKK1 Protein,hFc Tag

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详细介绍

性能参数

分子别名（Synonyms）	SK;Dkk1;Dickkopf-1
表达区间及表达系统（Source）	Human DKK1 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Thr32-His266.[Accession \| O94907]
分子量大小（Molecular Weight）	The protein has a predicted MW of 52.54 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human DKK1, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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