Recombinant Mouse C10/CCL6

Accession

P27784

GeneID

20305

Source

E.coli-derived Rhesus Macaque Eotaxin protein,Gly22-Ala116.

Molecular Weight

Approximately 10.7 kDa.

AA Sequence

GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/mL

Endotoxin

<1 EU/μg of protein as determined by LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.