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Recombinant Human LAG3/CD223(His Tag)

品牌：翌圣生物
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翌圣生物科技(上海)股份有限公司更新时间：2024-12-24 09:03:45

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详细介绍

LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling. Human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively. LAG-3 is expressed on activated CD4⁺ and CD8⁺ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4⁺CD25⁺ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli.

产品性质

别名（Synonyms）	CD223, FDC, LAG3, LAG-3, sLAG-3
Uniprot No.	P18627-1
表达区间及表达系统（Source）	HEK293 Cells-derived Human LAG3 / CD223 Protein, Leu23-Gly434 with His tag at the C-terminal
分子量(Molecular Weight)	Approximately 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	1.ELISA Data: Immobilized Human LAG3, His Tag at 0.5 μg/ml (100 μL/Well). Dose response curve for Anti-LAG3 Ab., hFc Tag with the EC₅₀ of 0.17 μg/mL determined by ELISA. 2.ELISA Data: Immobilized Human LAG3, His Tag at 1μg/mL (100 μL/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC₅₀ of 2.3 μg/mL determined by ELISA.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.