Recombinant Biotinylated Human DNAM-1/CD226 Protein,His-Avi Tag

表达区间及表达系统（Source）

Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu19--Asn247.[Accession | Q15762]

分子量大小（Molecular Weight）

The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and SEC-HPLC

活性（Activity）

SPR Data: Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.