Recombinant Biotinylated Human TPBG/5T4 Protein,His-Avi Tag

表达区间及表达系统（Source）

Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser32-Ser355.[Accession | Q13641-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 43.3ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.