Recombinant Human CD96/TACTILE Protein,His Tag

表达区间及表达系统（Source）

Recombinant Human CD96/TACTILE Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val22-Met503.[Accession | P40200-2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human CD96, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA   

SPR Data: Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 μM as determined in SPR assay 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.