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The detection of pesticide residues in tea
Abstract
Organic chlorine pesticide lipid solubility， easy in the food chain bioaccumulation， eventually entering the human body and human chronic toxicity， animal experiments to verify its teratogenic， carcinogenic effects. As the substitute of the organic chlorine pesticide， pyrethroid pesticides in agricultural production has been widely applied. This kind of pesticide application amount is small， easy degradation， residual period is shorter， the virus， pests， weeds， has strong killing effect， but this kind of pesticide also have certain accumulation， part of the human teratogenic， mutagenic effect. At present domestic and international tea organochlorine and pyrethroid pesticide detection by gas chromatography ( GC ) and gas chromatography - mass spectrometry ( GC-MS ). Sample of organochlorine and pyrethroid pesticides in organic solvents petroleum ether extraction， liquid liquid partition and chromatography purification， using petroleum ether and ethyl acetate (95+5) elution， collecting and purifying liquid， in a rotary evaporator concentration near the stem， petroleum ether constant volume， gas chromatography with electron capture detector， according to the retention time qualitative， external standard method for quantitative detection of organochlorine pesticides in tea， six six six， DDT and pyrethroid pesticides deltamethrin， permethrin， Cypermethrin， fenvalerate， Flucythrinate etc.. The method of the analysis results and test results are consistent. These pesticides in 0.0125-3.5000µg/mL range is linear， the method of adding the recovery rate was 81.71%-112.41%， this method has little solvent consumption， good purifying effect， simple operation， rapid， sensitive， accurate， reliable， for Guizhou area tea samples of Flucythrinate and so on many kinds of pesticide residues in need.
Key words: Tea gas chromatography organochlorine and synthetic pyrethroid pesticide residue