如何用原子力显微镜探测DNA与蛋白质间的相互左右

Large, multi-component protein assemblies are involved in many DNA transactions such as recombination, replication, transcription, and repair. In order to progress in the understanding of different key steps of these mechanisms, it is imperative to analyze the structure of the DNA-protein complexes involved and the dynamic interactions that govern their assembly, function and disassembly. These complexes are usually studied in vitro using a combination of biochemical and biophysical methods. A direct visualization technique such as AFM imaging is unique in that it allows one to characterize mechanisms involved in DNA-protein recognition and DNA-protein complex formation in different conditions (air and liquids of varying composition) with nanometer resolution. By comparison, other single molecule techniques such as optical or magnetic tweezers give indirect measurements based on the mechanical properties of single DNA molecules, and place severe restrictions on DNA size. Fluorescence microscopy can be used in conjunction with such approaches, but requires staining of either the DNA or the proteins and has relatively low resolution (about 200 nanometers). Owing to successive improvements to sample preparation and experimental methodology along with instrumentation progress, most notably the invention of TappingMode and the ability to operate in fluid AFM has indeed become a powerful and complementary tool to probe DNA-protein interactions at the single molecule level. The purpose of this application note is mainly to illustrate the potentialities of AFM imaging in the analysis of the architecture and dynamics of DNA-protein complexes, while introducing readers to the issue of DNA-protein complex adsorption, key for rationalizing sample preparation. DI Multimode V BioScope Catalyst 生物型原子力显微镜