HTRF combines time-resolved fluorescence (TRF) and fluorescence
resonance energy transfer (FRET). It is based on the energy
transfer between two fluorophores, a long-lived europium or
terbium cryptate donor and either a chemically modified allophycocyanine
(XL665) or a d2 acceptor. HTRF-based applications have
become increasingly popular for the analysis of various molecular
interactions and binding studies because of their homogeneity,
robustness, sensitivity, and potential for miniaturization.
The present application note summarizes the results of
experiments with the HTRF (Homogeneous Time-Resolved
Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO. Infinite® 200 Pro多功能微孔板检测仪
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