分解(Lysis)时间和其他变数对于新鲜罗勒的DNA萃取的影响

• Geno ApplicationNoteSP017(GG Basil DNA Extraction a comparative study) 分解(Lysis)时间和其他变数对于新鲜罗勒的DNA萃取的影响 A study was performed to discover what Geno/Grinder® processing variables significantly increased or decreased the yields of genomic DNA. Variables investigated included homogenization time and speed, while buffers, grinding media and tubes were constant. Comparison was done against another commercially available, cell lysing machine. In this experiment it was discovered that homogenization times of 90 seconds in the Geno/Grinder® at a speed of 2000 produces the best results. Conclusions If one chooses a method for processing fresh basil based solely on this experiment, the best choice would be 90 seconds in the Geno/Grinder® at a speed of 2000 RPM. We did not believe that an additional 30 seconds of processing time offered any benefit. It still may be possible to optimize the Geno/Grinder® at lower processing times (40-60 sec.) by changing the grinding media